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image lab software  (Bio-Rad)


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    Structured Review

    Bio-Rad image lab software
    Image Lab Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 36188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imaging+software/Image+Lab+Software/pmc12828604-99-4-7
    Average 99 stars, based on 36188 article reviews
    image lab software - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Labeling:

    Article Title: Proteomic insights uncover enhanced neurotherapeutic potential in conditioned mesenchymal stem cell-derived extracellular vesicles
    Article Snippet: Proteins were extracted from hippocampal samples and subjected to sodium dodecyl-sulfate polyacrylamide gel electrophoresis, transferred to polyvinylidene fluoride membranes, and probed with primary antibodies associated with inflammation and oxidation signaling, including NF-κB p65 (1:1000; 6956S, CST, MA, USA), NOX2 (1:2000; ab129068, Abcam, CA, USA), Nrf2 (1:1000; 16396-1-AP, Proteintech, IL, USA), and HO-1 (1:1000; 10701-1-AP, Proteintech, IL, USA), β-actin (1:100,000; AC026, Abclonal, Wuhan, China) was used as internal reference. .. Labeled proteins were detected and quantified using imaging software (Bio-Rad, Hercules, CA, USA). .. EV proteomics (n = 3 independent samples) and phosphoproteomics J. Xi et al. Extracellular Vesicle 3 (2024) 100037 (n = 3 independent samples) were performed separately, tandem mass tag (TMT)-based liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) identification and data normalization analysis were performed by PTM biolabTM (PTM20190820M02, Hangzhou, China) as previously described.21 EV samples were sonicated using a high intensity ultrasonic processor (Scientz, Ningbo, China) in lysis buffer and the remaining debris was removed by centrifugation (12,000 g × 10 min, 4 ◦C), the supernatant was collected and the protein concentration was determined with a BCA kit.

    Imaging:

    Article Title: Proteomic insights uncover enhanced neurotherapeutic potential in conditioned mesenchymal stem cell-derived extracellular vesicles
    Article Snippet: Proteins were extracted from hippocampal samples and subjected to sodium dodecyl-sulfate polyacrylamide gel electrophoresis, transferred to polyvinylidene fluoride membranes, and probed with primary antibodies associated with inflammation and oxidation signaling, including NF-κB p65 (1:1000; 6956S, CST, MA, USA), NOX2 (1:2000; ab129068, Abcam, CA, USA), Nrf2 (1:1000; 16396-1-AP, Proteintech, IL, USA), and HO-1 (1:1000; 10701-1-AP, Proteintech, IL, USA), β-actin (1:100,000; AC026, Abclonal, Wuhan, China) was used as internal reference. .. Labeled proteins were detected and quantified using imaging software (Bio-Rad, Hercules, CA, USA). .. EV proteomics (n = 3 independent samples) and phosphoproteomics J. Xi et al. Extracellular Vesicle 3 (2024) 100037 (n = 3 independent samples) were performed separately, tandem mass tag (TMT)-based liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) identification and data normalization analysis were performed by PTM biolabTM (PTM20190820M02, Hangzhou, China) as previously described.21 EV samples were sonicated using a high intensity ultrasonic processor (Scientz, Ningbo, China) in lysis buffer and the remaining debris was removed by centrifugation (12,000 g × 10 min, 4 ◦C), the supernatant was collected and the protein concentration was determined with a BCA kit.

    Article Title: Functional combination of resveratrol and midostaurin induces cytotoxicity to overcome acquired midostaurin resistance in FLT3-ITD expressing acute myeloid leukemia cells.
    Article Snippet: The most important challenge in treating FLT3-ITD AML is the development of resistance to FLT3 inhibitors, such as midostaurin, via both FLT3-dependent and FLT3-independent mechanisms.. The study explored the potential cytotoxic effects of combining resveratrol and midostaurin on the sensitization of midostaurin-resistant cells.. MTT assay revealed resveratrol’s chemo-sensitizing influence on midostaurin-resistant cells, and combination indexes (CI) were calculated using ChouTalalay’s method.

    Article Title: Tomatidine, a Steroidal Alkaloid, Synergizes with Cisplatin to Inhibit Cell Viability and Induce Cell Death Selectively on FLT3-ITD+ Acute Myeloid Leukemia Cells.
    Article Snippet: Background Acute Myeloid Leukemia (AML) is a hematological cancer that frequently presents with a range of side effects and drug resistance during anticancer drug treatment.. The current study aims to achieve increased efficacy by combining lower doses of cisplatin with increasing concentrations of tomatidine in AML cells to increase efficacy.. Methods Anti-proliferative effects of single and combination of cisplatin and tomatidine were assessed via MTT cell viability assay.

    Article Title: Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain
    Article Snippet: Finally, the membranes were cinubatee with an anti-rabbit secondary antibody (1:5000) conjugated with horseradish peroxidase (BBI, Nashville, TN, USA, Cat# D110058) and an anti-mouse secondary antibody (1:5000; BBI, Cat# D110085) for 2 hours at room temperature. .. The protein bands were detected and quantified using a Chemidoc XRS imager and laboratory imaging software (Bio-Rad, Hercules, CA, USA). .. Total protein values were normalized to histone H3 nucleoprotein (rabbit, 1:2000; Cell Signaling Technology, Cat# 4499, RRID: AB_10544537) and Tubulin (rabbit, 1:1000; ABclonal, Cat# AC015, RRID: AB_2773007).

    Article Title: Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain
    Article Snippet: Finally, the membranes were cinubatee with an anti-rabbit secondary antibody (1:5000) conjugated with horseradish peroxidase (BBI, Nashville, TN, USA, Cat# D110058) and an anti-mouse secondary antibody (1:5000; BBI, Cat# D110085) for 2 hours at room temperature. .. The protein bands were detected and quantified using a Chemidoc XRS imager and laboratory imaging software (Bio-Rad, Hercules, CA, USA). .. Total protein values were normalized to histone H3 nucleoprotein (rabbit, 1:2000; Cell Signaling Technology, Cat# 4499, RRID: AB_10544537) and Tubulin (rabbit, 1:1000; ABclonal, Cat# AC015, RRID: AB_2773007).

    Article Title: Effect of sulforaphane on testicular ischemia-reperfusion injury induced by testicular torsion-detorsion in rats
    Article Snippet: Finally, the protein immunoblots on the membrane were identified via an enhanced chemiluminescence Western blotting detection kit (Santa Cruz Biotechnology) according to protocols of the manufacturer. .. Analysis of the protein blot intensity was performed by the imaging software (Bio-Rad Laboratories). ..

    Article Title: Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain
    Article Snippet: The specific antibodies used in this experiments were as follows: antiPRMT6 (rabbit, 1:200; Novus Biologicals, Cat# NB100-56642, RRID: AB_838734), anti-hnRNP-F (mouse, 1:200; Thermo Fisher Scientific, Cat# MA5-18024, RRID: AB_2539408), antiFlag (rabbit, 2 μg; Proteintech, Wuhan, China, Cat# 80010-1- RR, RRID: AB_2882940), anti-His (rabbit, 1:1000; Proteintech, Cat# 10001-0-AP, RRID: AB_11232228), and anti-β-tubulin (rabbit, 1:1000; ABclonal, Cat# AC015, RRID: AB_2773007). .. The bands were detected and quantified using a Chemidoc XRS imager and laboratory imaging software (Bio-Rad). .. Beijing Bio-Tech Pack Technology Company (Beijing, China) performed the label-free proteomics experiments.

    Article Title: Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain
    Article Snippet: The specific antibodies used in this experiments were as follows: anti-PRMT6 (rabbit, 1:200; Novus Biologicals, Cat# NB100-56642, RRID: AB_838734), anti-hnRNP-F (mouse, 1:200; Thermo Fisher Scientific, Cat# MA5-18024, RRID: AB_2539408), anti-Flag (rabbit, 2 μg; Proteintech, Wuhan, China, Cat# 80010-1-RR, RRID: AB_2882940), anti-His (rabbit, 1:1000; Proteintech, Cat# 10001-0-AP, RRID: AB_11232228), and anti-β-tubulin (rabbit, 1:1000; ABclonal, Cat# AC015, RRID: AB_2773007). .. The bands were detected and quantified using a Chemidoc XRS imager and laboratory imaging software (Bio-Rad). .. Beijing Bio-Tech Pack Technology Company (Beijing, China) performed the label-free proteomics experiments.

    Software:

    Article Title: Proteomic insights uncover enhanced neurotherapeutic potential in conditioned mesenchymal stem cell-derived extracellular vesicles
    Article Snippet: Proteins were extracted from hippocampal samples and subjected to sodium dodecyl-sulfate polyacrylamide gel electrophoresis, transferred to polyvinylidene fluoride membranes, and probed with primary antibodies associated with inflammation and oxidation signaling, including NF-κB p65 (1:1000; 6956S, CST, MA, USA), NOX2 (1:2000; ab129068, Abcam, CA, USA), Nrf2 (1:1000; 16396-1-AP, Proteintech, IL, USA), and HO-1 (1:1000; 10701-1-AP, Proteintech, IL, USA), β-actin (1:100,000; AC026, Abclonal, Wuhan, China) was used as internal reference. .. Labeled proteins were detected and quantified using imaging software (Bio-Rad, Hercules, CA, USA). .. EV proteomics (n = 3 independent samples) and phosphoproteomics J. Xi et al. Extracellular Vesicle 3 (2024) 100037 (n = 3 independent samples) were performed separately, tandem mass tag (TMT)-based liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) identification and data normalization analysis were performed by PTM biolabTM (PTM20190820M02, Hangzhou, China) as previously described.21 EV samples were sonicated using a high intensity ultrasonic processor (Scientz, Ningbo, China) in lysis buffer and the remaining debris was removed by centrifugation (12,000 g × 10 min, 4 ◦C), the supernatant was collected and the protein concentration was determined with a BCA kit.

    Article Title: Functional combination of resveratrol and midostaurin induces cytotoxicity to overcome acquired midostaurin resistance in FLT3-ITD expressing acute myeloid leukemia cells.
    Article Snippet: The most important challenge in treating FLT3-ITD AML is the development of resistance to FLT3 inhibitors, such as midostaurin, via both FLT3-dependent and FLT3-independent mechanisms.. The study explored the potential cytotoxic effects of combining resveratrol and midostaurin on the sensitization of midostaurin-resistant cells.. MTT assay revealed resveratrol’s chemo-sensitizing influence on midostaurin-resistant cells, and combination indexes (CI) were calculated using ChouTalalay’s method.

    Article Title: Tomatidine, a Steroidal Alkaloid, Synergizes with Cisplatin to Inhibit Cell Viability and Induce Cell Death Selectively on FLT3-ITD+ Acute Myeloid Leukemia Cells.
    Article Snippet: Background Acute Myeloid Leukemia (AML) is a hematological cancer that frequently presents with a range of side effects and drug resistance during anticancer drug treatment.. The current study aims to achieve increased efficacy by combining lower doses of cisplatin with increasing concentrations of tomatidine in AML cells to increase efficacy.. Methods Anti-proliferative effects of single and combination of cisplatin and tomatidine were assessed via MTT cell viability assay.

    Article Title: Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain
    Article Snippet: Finally, the membranes were cinubatee with an anti-rabbit secondary antibody (1:5000) conjugated with horseradish peroxidase (BBI, Nashville, TN, USA, Cat# D110058) and an anti-mouse secondary antibody (1:5000; BBI, Cat# D110085) for 2 hours at room temperature. .. The protein bands were detected and quantified using a Chemidoc XRS imager and laboratory imaging software (Bio-Rad, Hercules, CA, USA). .. Total protein values were normalized to histone H3 nucleoprotein (rabbit, 1:2000; Cell Signaling Technology, Cat# 4499, RRID: AB_10544537) and Tubulin (rabbit, 1:1000; ABclonal, Cat# AC015, RRID: AB_2773007).

    Article Title: Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain
    Article Snippet: Finally, the membranes were cinubatee with an anti-rabbit secondary antibody (1:5000) conjugated with horseradish peroxidase (BBI, Nashville, TN, USA, Cat# D110058) and an anti-mouse secondary antibody (1:5000; BBI, Cat# D110085) for 2 hours at room temperature. .. The protein bands were detected and quantified using a Chemidoc XRS imager and laboratory imaging software (Bio-Rad, Hercules, CA, USA). .. Total protein values were normalized to histone H3 nucleoprotein (rabbit, 1:2000; Cell Signaling Technology, Cat# 4499, RRID: AB_10544537) and Tubulin (rabbit, 1:1000; ABclonal, Cat# AC015, RRID: AB_2773007).

    Article Title: Effect of sulforaphane on testicular ischemia-reperfusion injury induced by testicular torsion-detorsion in rats
    Article Snippet: Finally, the protein immunoblots on the membrane were identified via an enhanced chemiluminescence Western blotting detection kit (Santa Cruz Biotechnology) according to protocols of the manufacturer. .. Analysis of the protein blot intensity was performed by the imaging software (Bio-Rad Laboratories). ..

    Article Title: Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain
    Article Snippet: The specific antibodies used in this experiments were as follows: antiPRMT6 (rabbit, 1:200; Novus Biologicals, Cat# NB100-56642, RRID: AB_838734), anti-hnRNP-F (mouse, 1:200; Thermo Fisher Scientific, Cat# MA5-18024, RRID: AB_2539408), antiFlag (rabbit, 2 μg; Proteintech, Wuhan, China, Cat# 80010-1- RR, RRID: AB_2882940), anti-His (rabbit, 1:1000; Proteintech, Cat# 10001-0-AP, RRID: AB_11232228), and anti-β-tubulin (rabbit, 1:1000; ABclonal, Cat# AC015, RRID: AB_2773007). .. The bands were detected and quantified using a Chemidoc XRS imager and laboratory imaging software (Bio-Rad). .. Beijing Bio-Tech Pack Technology Company (Beijing, China) performed the label-free proteomics experiments.

    Article Title: Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain
    Article Snippet: The specific antibodies used in this experiments were as follows: anti-PRMT6 (rabbit, 1:200; Novus Biologicals, Cat# NB100-56642, RRID: AB_838734), anti-hnRNP-F (mouse, 1:200; Thermo Fisher Scientific, Cat# MA5-18024, RRID: AB_2539408), anti-Flag (rabbit, 2 μg; Proteintech, Wuhan, China, Cat# 80010-1-RR, RRID: AB_2882940), anti-His (rabbit, 1:1000; Proteintech, Cat# 10001-0-AP, RRID: AB_11232228), and anti-β-tubulin (rabbit, 1:1000; ABclonal, Cat# AC015, RRID: AB_2773007). .. The bands were detected and quantified using a Chemidoc XRS imager and laboratory imaging software (Bio-Rad). .. Beijing Bio-Tech Pack Technology Company (Beijing, China) performed the label-free proteomics experiments.



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